Skip to content

flowjo_to_gatingset(), colname not found: error #148

Description

@KammannT

Hi,
I am struggling to parse a flowjo.wsp file containing all the compensation and gating for my fcs files.

library(CytoML)
fcs_dir <- 'PathtoFolderoffcsfiles'
wsp_file <- 'PathtoFlowjowspfile'
flow <- CytoML::open_flowjo_xml(wsp_file)

The workspace can be loaded and indicates number of samples, groups, keywords, etc.

test <-CytoML::flow_to_gatingset(flow, path=fcs_dir, name=1)

line above gives the error:
"Error in (function (ws, group_id, subset, execute, path, cytoset, backend_dir, :
colname not found: Comp-BV605-CD161"

Loading a .xml file of the same Flowjo workspace (instead of .wsp) gives a different error but can however save the file:

xml_file <- 'PathtoFlowjoxmlfile'
flow2 <- CytoML::open_flowjo_xml(xml_file)

Error message:
^namespace error: Namespace prefix data-type on fas-dimension is not defined
<data-type:fcs-dimension data-type:name="Comp-BUV737-CD69"^

test2 <-CytoML::flow_to_gatingset(flow2, path=fcs_dir, name=1)

line above gives the error:
Error in (function (ws, group_id, subset, execute, path, cytoset, backend_dir, :
*: unknown tranformation type!transforms:linear

Support is very much appreciated! Thanks

Activity

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Type

    No type

    Projects

    No projects

      Milestone

      No milestone

      Relationships

      None yet

      Development

      No branches or pull requests

      Issue actions